Analysis

1) There are currently six web pages under the analysis menu: Detailed analysis, Interactions, Side-by-side comparison, Predict pathogenicity, Gene credibility and Interactive Molecular stucture.

2) Clicking on the Detailed analysis submenu, an automatically populated checkbox list of genes with patient data, two dropdownlists on age of onset range (in years) from 0 to 100 and radiobuttons for further selections on gender, inheritance type and site of onset.

3) Clicking on the Interactions submenu, a checkboxlist of all available genes in ALS are displayed. A group of checkboxes dynamically derived from the gene table is displayed to allow users select as many gene interactions they would like to analyse. Once the genes are selected, the list is presented as confirmation. The “Execute” link is clicked to display the prediction on the same page. If the “Reset” link is clicked, all the selected boxes are automatically deselected and the graphical analysis is cleared to allow for fresh analysis.

4) Clicking on the Side-by-side submenu, runs a query analysis on genetic data displaying 4 dropdownlists to select any two genes and the age range of patients. A side-by-side comparison of mutation and patient data on two selected genes is submitted to the database. The query is analysed and displayed graphically on the website automatically in a split of a second. It shows the site of onset, mean age using box plot, gender, family history, nationalities of patients and other joint analysis of both genes.

5) Clicking on the Predict pathogenicity submenu, a list of genes are automatically pooled from the database based on the availability of genes with at least a substitution mutation.

6) Clicking on the Gene credibility submenu, 11 checkboxes appear as user-configurable criteria.

6) Clicking on the Molecular structure displays an interactive image for users to view by right clicking on the image.